Biochemistry Syllabus PDF | B.Pharm 2nd Semester | Old Syllabus

Biochemistry syllabus for 2nd Semester. This page presents the detailed syllabus content from the original Old Syllabus PDF.

About This Syllabus

• Biomolecules
Introduction, classification, chemical nature and biological role of
carbohydrate, lipids, nucleic acids, amino acids and proteins.

Course Syllabus

Unit I • Biomolecules

Introduction, classification, chemical nature and biological role of

carbohydrate, lipids, nucleic acids, amino acids and proteins.

• Bioenergetics

Concept of free energy, endergonic and exergonic reaction, Relationship between free energy, enthalpy and entropy; Redox potential. Energy rich compounds; classification; biological significances of ATP and cyclic AMP

Unit Ii • Carbohydrate Metabolism

Glycolysis – Pathway, energetics and significance Citric acid cycle- Pathway, energetics and significance HMP shunt and its significance; Glucose-6-Phosphate dehydrogenase (G6PD) deficiency Glycogen metabolism Pathways and glycogen storage diseases (GSD) Gluconeogenesis- Pathway and its significance Hormonal regulation of blood glucose level and Diabetes mellitus

• Biological oxidation

Electron transport chain (ETC) and its mechanism. Oxidative phosphorylation & its mechanism and substrate phosphorylation Inhibitors ETC and oxidative phosphorylation/Uncouplers level

Unit Iii • Lipid Metabolism

β-Oxidation of saturated fatty acid (Palmitic acid) Formation and utilization of ketone bodies; ketoacidosis De novo synthesis of fatty acids (Palmitic acid) Biological significance of cholesterol and conversion of cholesterol into bile acids, steroid hormone and vitamin D Disorders of lipid metabolism: Hypercholesterolemia, atherosclerosis, fatty liver and obesity.

• Amino acid metabolism

General reactions of amino acid metabolism: Transamination, deamination & decarboxylation, urea cycle and its disorders Catabolism of phenylalanine and tyrosine and their metabolic disorders (Phenyketonuria, Albinism, alkeptonuria, tyrosinemia) Synthesis and significance of biological substances; 5-HT, melatonin, dopamine, noradrenaline, adrenaline Catabolism of heme; hyperbilirubinemia and jaundice

Unit Iv • Nucleic Acid Metabolism And Genetic Information Transfer

Biosynthesis of purine and pyrimidine nucleotides Catabolism of purine nucleotides and Hyperuricemia and Gout disease Organization of mammalian genome Structure of DNA and RNA and their functions DNA replication (semi conservative model) Transcription or RNA synthesis Genetic code, Translation or Protein synthesis and inhibitors

Unit V • Enzymes

Introduction, properties, nomenclature and IUB classification of enzymes

Enzyme kinetics (Michaelis plot, Line Weaver Burke plot) Enzyme inhibitors with examples Regulation of enzymes: enzyme induction and repression, allosteric enzymes regulation Therapeutic and diagnostic applications of enzymes and isoenzymes Coenzymes –Structure and biochemical functions BP 209 P. BIOCHEMISTRY (Practical) 1. Qualitative analysis of carbohydrates (Glucose, Fructose, Lactose, Maltose, Sucrose and starch) 2. Identification tests for Proteins (albumin and Casein) 3. Quantitative analysis of reducing sugars (DNSA method) and Proteins (Biuret method) 4. Qualitative analysis of urine for abnormal constituents 5. Determination of blood creatinine

6. Determination of blood sugar 7. Determination of serum total cholesterol 8. Preparation of buffer solution and measurement of pH 9. Study of enzymatic hydrolysis of starch 10. Determination of Salivary amylase activity 11. Study the effect of Temperature on Salivary amylase activity. 12. Study the effect of substrate concentration on salivary amylase activity.

Practical Syllabus

1. Qualitative analysis of carbohydrates (Glucose, Fructose, Lactose, Maltose,
2. Identification tests for Proteins (albumin and Casein)
3. Quantitative analysis of reducing sugars (DNSA method) and Proteins
4. Qualitative analysis of urine for abnormal constituents
5. Determination of blood creatinine
6. Determination of blood sugar
7. Determination of serum total cholesterol
8. Preparation of buffer solution and measurement of pH
9. Study of enzymatic hydrolysis of starch
10. Determination of Salivary amylase activity
11. Study the effect of Temperature on Salivary amylase activity.
12. Study the effect of substrate concentration on salivary amylase activity.

The original syllabus PDF is available below for students who want to download and refer to the complete document.