Instrumental Syllabus PDF | B.Pharm 7th Semester | Old Syllabus

Instrumental Methods of Analysis is a B.Pharm 7th Semester subject dealing with the application of instrumental methods in qualitative and quantitative analysis of drugs. It covers the principles and instrumentation of spectroscopic and chromatographic techniques used in drug testing.

About This Syllabus

This subject deals with the application of instrumental methods in qualitative and quantitative analysis of drugs. It provides fundamental knowledge of spectroscopic and chromatographic techniques and their application in pharmaceutical analysis.

Course Syllabus

Unit I – Spectroscopic Methods

UV-Visible Spectroscopy

• Electronic transitions
• Chromophores
• Auxochromes
• Spectral shifts
• Solvent effect on absorption spectra
• Beer and Lambert’s law
• Derivation and deviations
• Instrumentation: sources of radiation, wavelength selectors, sample cells and detectors
• Phototube
• Photomultiplier tube
• Photovoltaic cell
• Silicon photodiode
• Applications: spectrophotometric titrations
• Single-component and multicomponent analysis

Fluorescence Spectroscopy

• Theory
• Concepts of singlet, doublet and triplet electronic states
• Internal and external conversions
• Factors affecting fluorescence
• Quenching
• Instrumentation and applications

Infrared Spectroscopy

• Fundamental modes of vibrations in polyatomic molecules
• Sample handling
• Factors affecting vibrations
• Instrumentation
• Sources of radiation
• Wavelength selectors
• Detectors including Golay cell, thermocouple, thermistor and pyroelectric detector
• Applications

Atomic Absorption Spectroscopy

• Principle
• Interferences
• Instrumentation
• Applications

Flame Photometry

• Principle
• Instrumentation
• Applications

Unit II – Chromatographic and Electrophoretic Methods

Introduction to Chromatography

• Adsorption and partition column chromatography
• Methodology
• Advantages and disadvantages
• Applications

Thin Layer Chromatography

• Introduction
• Principle
• Methodology
• Rf values
• Advantages and disadvantages
• Applications

Paper Chromatography

• Introduction
• Methodology
• Development techniques
• Advantages and disadvantages
• Applications

Electrophoresis

• Introduction
• Factors affecting electrophoretic mobility
• Paper electrophoresis
• Gel electrophoresis
• Capillary electrophoresis
• Applications

Unit III – Gas Chromatography and HPLC

Gas Chromatography

• Introduction
• Theory
• Instrumentation
• Derivatization
• Temperature programming
• Advantages and disadvantages
• Applications

High Performance Liquid Chromatography (HPLC)

• Introduction
• Theory
• Instrumentation
• Advantages
• Applications

Unit IV – Ion Exchange, Gel and Affinity Chromatography

Ion Exchange Chromatography

• Introduction
• Classification
• Ion exchange resins
• Properties
• Mechanism of ion exchange process
• Factors affecting ion exchange
• Methodology
• Applications

Gel Chromatography

• Introduction
• Theory
• Instrumentation
• Applications

Affinity Chromatography

• Introduction
• Theory
• Instrumentation
• Applications

About This Syllabus

This page provides the Instrumental Methods of Analysis syllabus for B.Pharm 7th Semester. The syllabus covers spectroscopic methods, chromatographic techniques and electrophoresis used in pharmaceutical and drug analysis.

The original syllabus PDF is available below for students who want to download and refer to the complete document.