Pharmaceutical Micrology 3rd Syllabus PDF | B.Pharm 3rd Semester | Old Syllabus

Pharmaceutical Micrology 3rd syllabus for 3rd Semester. This page presents the detailed syllabus content from the original Old Syllabus PDF.

About This Syllabus

Introduction, history of microbiology, its branches, scope and its
importance.

Course Syllabus

Unit I Introduction, History Of Microbiology, Its Branches, Scope And Its

importance.

Introduction to Prokaryotes and Eukaryotes

Study of ultra-structure and morphological classification of bacteria, nutritional requirements, raw materials used for culture media and physical parameters for growth, growth curve, isolation and preservation methods for pure cultures, cultivation of anaerobes, quantitative measurement of bacterial growth (total & viable count). Study of different types of phase constrast microscopy, dark field microscopy and electron microscopy.

Unit Ii Identification Of Bacteria Using Staining Techniques (Simple, Gram’S &Acid

fast staining) and biochemical tests (IMViC). Study of principle, procedure, merits, demerits and applications of physical, chemical gaseous,radiation and mechanical method of sterilization. Evaluation of the efficiency of sterilization methods. Equipments employed in large scale sterilization. Sterility indicators.

Unit Iii Study Of Morphology, Classification, Reproduction/Replication And

cultivation of Fungi and Viruses. Classification and mode of action of disinfectants Factors influencing disinfection, antiseptics and their evaluation. For bacteriostatic and bactericidal actions Evaluation of bactericidal & Bacteriostatic. Sterility testing of products (solids, liquids, ophthalmic and other sterile products) according to IP, BP and USP.

Unit Iv Designing Of Aseptic Area, Laminar Flow Equipments; Study Of Different

sources of contamination in an aseptic area and methods of prevention, clean area classification. Principles and methods of different microbiological assay. Methods for standardization of antibiotics, vitamins and amino acids. Assessment of a new antibiotic.

Unit V Types Of Spoilage, Factors Affecting The Microbial Spoilage Of

pharmaceutical products, sources and types of microbial contaminants, assessment of microbial contamination and spoilage. Preservation of pharmaceutical products using antimicrobial agents, evaluation of microbial stability of formulations. Growth of animal cells in culture, general procedure for cell culture, Primary, established and transformed cell cultures. Application of cell cultures in pharmaceutical industry and research. BP 307P.PHARMACEUTICAL MICROBIOLOGY (Practical) 1. Introduction and study of different equipments and processing, e.g., B.O.D. incubator, laminar flow, aseptic hood, autoclave, hot air sterilizer, deep freezer, refrigerator,

microscopes used in experimental microbiology. 2. Sterilization of glassware, preparation and sterilization of media. 3. Sub culturing of bacteria and fungus. Nutrient stabs and slants preparations. 4. Staining methods- Simple, Grams staining and acid fast staining (Demonstration with

practical).

5. Isolation of pure culture of micro-organisms by multiple streak plate technique and other techniques. 6. Microbiological assay of antibiotics by cup plate method and other methods 7. Motility determination by Hanging drop method. 8. Sterility testing of pharmaceuticals. 9. Bacteriological analysis of water 10. Biochemical test.

Practical Syllabus

1. Introduction and study of different equipments and processing, e.g., B.O.D.
2. Sterilization of glassware, preparation and sterilization of media.
3. Sub culturing of bacteria and fungus. Nutrient stabs and slants preparations.
4. Staining methods- Simple, Grams staining and acid fast staining
5. Isolation of pure culture of micro-organisms by multiple streak plate
6. Microbiological assay of antibiotics by cup plate method and other methods
7. Motility determination by Hanging drop method.
8. Sterility testing of pharmaceuticals.
9. Bacteriological analysis of water
10. Biochemical test.

The original syllabus PDF is available below for students who want to download and refer to the complete document.