UV–Visible Spectrophotometric Analysis
Standard pharmaceutical calculation, laboratory analysis and research tools.
Analyze UV–Visible spectrophotometric data using absorbance, concentration and calibration relationships commonly applied in pharmaceutical analysis.
What Is UV–Visible Spectrophotometry?
UV–Visible spectrophotometry measures the absorption of ultraviolet or visible radiation by a sample and is widely used for quantitative pharmaceutical analysis.
Beer–Lambert Law
The Beer–Lambert relationship is commonly expressed as A = εbc, where A is absorbance, ε is molar absorptivity, b is path length and c is concentration.
Absorbance and Concentration
Within an appropriate linear analytical range, absorbance is proportional to concentration. This relationship allows concentration to be estimated from measured absorbance.
Calibration Curve
A calibration curve is prepared using standards of known concentration and corresponding absorbance values. The resulting relationship can be used to estimate unknown concentration.
Pharmaceutical Applications
UV–Visible analysis is used in assay determination, dissolution analysis, content estimation, analytical method development and pharmaceutical quality-control testing.
Frequently Asked Questions
What is Beer–Lambert law?
It relates absorbance to path length, molar absorptivity and concentration under appropriate analytical conditions.
What does absorbance indicate?
Absorbance represents the amount of incident radiation absorbed by the sample at a selected wavelength.
Why is a calibration curve used?
It establishes the relationship between known concentrations and measured responses so unknown concentrations can be estimated.